Construct: shRNA TRCN0000079022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_486490.1-914s1c1
DNA Barcode:
CATCGTACAGCCATGATAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC385186 (385186)
Gene Description:
similar to Aurkc protein
Transcript:
RefSeq XM_486490.1 (NON-CURRENT)
Match location:
Position 914 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGTACAGCCATGATAATA
Hairpin Sequence:
5'-CCGG-CATCGTACAGCCATGATAATA-CTCGAG-TATTATCATGGCTGTACGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGTACAGCCATGATAATACTCGAGTATTATCATGGCTGTACGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGTACAGCCATGATAATACTCGAGTATTATCATGGCTGTACGATG-3'

Other clones with same target sequence:

(none)