Construct: shRNA TRCN0000079033

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010279.2-1997s1c1
DNA Barcode:
GCAGACTCTATCTTGTACTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gfra1 (14585)
Gene Description:
glial cell line derived neurotrophic factor family receptor alpha 1
Transcript:
RefSeq NM_010279.2 (NON-CURRENT)
Match location:
Position 1997 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079033)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGACTCTATCTTGTACTAA
Hairpin Sequence:
5'-CCGG-GCAGACTCTATCTTGTACTAA-CTCGAG-TTAGTACAAGATAGAGTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGACTCTATCTTGTACTAACTCGAGTTAGTACAAGATAGAGTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGACTCTATCTTGTACTAACTCGAGTTAGTACAAGATAGAGTCTGC-3'

Other clones with same target sequence:

(none)