Construct: shRNA TRCN0000079038

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001983.1-6236s1c1
DNA Barcode:
GCCTTGAGTGTCACAGAGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pi4ka (224020)
Gene Description:
phosphatidylinositol 4-kinase alpha
Transcript:
RefSeq NM_001001983.1 (NON-CURRENT)
Match location:
Position 6236 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079038)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGAGTGTCACAGAGCAA
Hairpin Sequence:
5'-CCGG-GCCTTGAGTGTCACAGAGCAA-CTCGAG-TTGCTCTGTGACACTCAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGAGTGTCACAGAGCAACTCGAGTTGCTCTGTGACACTCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGAGTGTCACAGAGCAACTCGAGTTGCTCTGTGACACTCAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000311975