Construct: shRNA TRCN0000079082

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153587.1-1767s1c1
DNA Barcode:
CCATGCCTGAAATTGTGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rps6ka5 (73086)
Gene Description:
ribosomal protein S6 kinase, polypeptide 5
Transcript:
RefSeq NM_153587.1 (NON-CURRENT)
Match location:
Position 1767 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079082)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGCCTGAAATTGTGCTAT
Hairpin Sequence:
5'-CCGG-CCATGCCTGAAATTGTGCTAT-CTCGAG-ATAGCACAATTTCAGGCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGCCTGAAATTGTGCTATCTCGAGATAGCACAATTTCAGGCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGCCTGAAATTGTGCTATCTCGAGATAGCACAATTTCAGGCATGG-3'

Other clones with same target sequence:

(none)