Construct: shRNA TRCN0000079107

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029426.1-2159s1c1
DNA Barcode:
CTACTCAGTCACATTCACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Brsk2 (75770)
Gene Description:
BR serine/threonine kinase 2
Transcript:
RefSeq NM_029426.1 (NON-CURRENT)
Match location:
Position 2159 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079107)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACTCAGTCACATTCACTTT
Hairpin Sequence:
5'-CCGG-CTACTCAGTCACATTCACTTT-CTCGAG-AAAGTGAATGTGACTGAGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACTCAGTCACATTCACTTTCTCGAGAAAGTGAATGTGACTGAGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACTCAGTCACATTCACTTTCTCGAGAAAGTGAATGTGACTGAGTAG-3'

Other clones with same target sequence:

(none)