Construct: shRNA TRCN0000079160

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026229.1-346s1c1
DNA Barcode:
CCCTTCTACATTGGTTACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr89 (67549)
Gene Description:
G protein-coupled receptor 89
Transcript:
RefSeq NM_026229.1 (NON-CURRENT)
Match location:
Position 346 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079160)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTCTACATTGGTTACTTT
Hairpin Sequence:
5'-CCGG-CCCTTCTACATTGGTTACTTT-CTCGAG-AAAGTAACCAATGTAGAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTCTACATTGGTTACTTTCTCGAGAAAGTAACCAATGTAGAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTCTACATTGGTTACTTTCTCGAGAAAGTAACCAATGTAGAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000326032