Construct: shRNA TRCN0000079207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_205565.3-2049s1c1
DNA Barcode:
CTGCTGCATGTTTGACCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rsph10b (75136)
Gene Description:
radial spoke head 10 homolog B (Chlamydomonas)
Transcript:
RefSeq XM_205565.3 (NON-CURRENT)
Match location:
Position 2049 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCTGCATGTTTGACCAGAT
Hairpin Sequence:
5'-CCGG-CTGCTGCATGTTTGACCAGAT-CTCGAG-ATCTGGTCAAACATGCAGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCTGCATGTTTGACCAGATCTCGAGATCTGGTCAAACATGCAGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCTGCATGTTTGACCAGATCTCGAGATCTGGTCAAACATGCAGCAG-3'

Other clones with same target sequence:

(none)