Construct: shRNA TRCN0000079216

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_129976.2-2153s1c1
DNA Barcode:
GCAATTCAGATACATGATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slco6c1 (74441)
Gene Description:
solute carrier organic anion transporter family, member 6c1
Transcript:
RefSeq XM_129976.2 (NON-CURRENT)
Match location:
Position 2153 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079216)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATTCAGATACATGATGTT
Hairpin Sequence:
5'-CCGG-GCAATTCAGATACATGATGTT-CTCGAG-AACATCATGTATCTGAATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATTCAGATACATGATGTTCTCGAGAACATCATGTATCTGAATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATTCAGATACATGATGTTCTCGAGAACATCATGTATCTGAATTGC-3'

Other clones with same target sequence:

(none)