Construct: shRNA TRCN0000079224

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033552.1-187s1c1
DNA Barcode:
CGGACATTATTTATTGGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc4a10 (94229)
Gene Description:
solute carrier family 4, sodium bicarbonate cotransporter-like, member 10
Transcript:
RefSeq NM_033552.1 (NON-CURRENT)
Match location:
Position 187 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079224)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACATTATTTATTGGAGTT
Hairpin Sequence:
5'-CCGG-CGGACATTATTTATTGGAGTT-CTCGAG-AACTCCAATAAATAATGTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACATTATTTATTGGAGTTCTCGAGAACTCCAATAAATAATGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACATTATTTATTGGAGTTCTCGAGAACTCCAATAAATAATGTCCG-3'

Other clones with same target sequence:

(none)