Construct: shRNA TRCN0000079224
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_033552.1-187s1c1
- DNA Barcode:
- CGGACATTATTTATTGGAGTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Slc4a10 (94229)
- Gene Description:
- solute carrier family 4, sodium bicarbonate cotransporter-like, member 10
- Transcript:
- RefSeq NM_033552.1 (NON-CURRENT)
- Match location:
- Position 187 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000079224)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGGACATTATTTATTGGAGTT
- Hairpin Sequence:
-
5'-CCGG-CGGACATTATTTATTGGAGTT-CTCGAG-AACTCCAATAAATAATGTCCG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGGACATTATTTATTGGAGTTCTCGAGAACTCCAATAAATAATGTCCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGGACATTATTTATTGGAGTTCTCGAGAACTCCAATAAATAATGTCCG-3'
Other clones with same target sequence:
(none)
