Construct: shRNA TRCN0000079235

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175316.2-343s1c1
DNA Barcode:
GCTTTCAATGAGGTAGGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slco2b1 (101488)
Gene Description:
solute carrier organic anion transporter family, member 2b1
Transcript:
RefSeq NM_175316.2 (NON-CURRENT)
Match location:
Position 343 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079235)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCAATGAGGTAGGGAAT
Hairpin Sequence:
5'-CCGG-GCTTTCAATGAGGTAGGGAAT-CTCGAG-ATTCCCTACCTCATTGAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCAATGAGGTAGGGAATCTCGAGATTCCCTACCTCATTGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCAATGAGGTAGGGAATCTCGAGATTCCCTACCTCATTGAAAGC-3'

Other clones with same target sequence:

(none)