Construct: shRNA TRCN0000079286
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_134251.1-462s1c1
- DNA Barcode:
- GCGTGGAATTTCAGTTGCTGT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Slc12a8 (171286)
- Gene Description:
- solute carrier family 12 (potassium/chloride transporters), member 8
- Transcript:
- RefSeq NM_134251.1 (NON-CURRENT)
- Match location:
- Position 462 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000079286)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCGTGGAATTTCAGTTGCTGT
- Hairpin Sequence:
-
5'-CCGG-GCGTGGAATTTCAGTTGCTGT-CTCGAG-ACAGCAACTGAAATTCCACGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCGTGGAATTTCAGTTGCTGTCTCGAGACAGCAACTGAAATTCCACGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCGTGGAATTTCAGTTGCTGTCTCGAGACAGCAACTGAAATTCCACGC-3'
Other clones with same target sequence:
(none)
