Construct: shRNA TRCN0000079312

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009209.2-612s1c1
DNA Barcode:
CCATACCAAATACTCCAAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc6a2 (20538)
Gene Description:
solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2
Transcript:
RefSeq NM_009209.2 (NON-CURRENT)
Match location:
Position 612 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079312)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATACCAAATACTCCAAATA
Hairpin Sequence:
5'-CCGG-CCATACCAAATACTCCAAATA-CTCGAG-TATTTGGAGTATTTGGTATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATACCAAATACTCCAAATACTCGAGTATTTGGAGTATTTGGTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATACCAAATACTCCAAATACTCGAGTATTTGGAGTATTTGGTATGG-3'

Other clones with same target sequence:

(none)