Construct: shRNA TRCN0000079333

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004184.1-2261s1c1
DNA Barcode:
CCTCTCTATTCCTCTTTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc28a1 (434203)
Gene Description:
solute carrier family 28 (sodium-coupled nucleoside transporter), member 1
Transcript:
RefSeq NM_001004184.1 (NON-CURRENT)
Match location:
Position 2261 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079333)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCTATTCCTCTTTCTTT
Hairpin Sequence:
5'-CCGG-CCTCTCTATTCCTCTTTCTTT-CTCGAG-AAAGAAAGAGGAATAGAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCTATTCCTCTTTCTTTCTCGAGAAAGAAAGAGGAATAGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCTATTCCTCTTTCTTTCTCGAGAAAGAAAGAGGAATAGAGAGG-3'

Other clones with same target sequence:

(none)