Construct: shRNA TRCN0000079334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004184.1-359s1c1
DNA Barcode:
CCTGCTCTCTACTGCATACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc28a1 (434203)
Gene Description:
solute carrier family 28 (sodium-coupled nucleoside transporter), member 1
Transcript:
RefSeq NM_001004184.1 (NON-CURRENT)
Match location:
Position 359 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTCTCTACTGCATACAT
Hairpin Sequence:
5'-CCGG-CCTGCTCTCTACTGCATACAT-CTCGAG-ATGTATGCAGTAGAGAGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTCTCTACTGCATACATCTCGAGATGTATGCAGTAGAGAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTCTCTACTGCATACATCTCGAGATGTATGCAGTAGAGAGCAGG-3'

Other clones with same target sequence:

(none)