Construct: shRNA TRCN0000079414

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130451.1-1575s1c1
DNA Barcode:
GCTGAAATAGAGCAGCAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc2a10 (170441)
Gene Description:
solute carrier family 2 (facilitated glucose transporter), member 10
Transcript:
RefSeq NM_130451.1 (NON-CURRENT)
Match location:
Position 1575 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079414)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAATAGAGCAGCAGTTT
Hairpin Sequence:
5'-CCGG-GCTGAAATAGAGCAGCAGTTT-CTCGAG-AAACTGCTGCTCTATTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAATAGAGCAGCAGTTTCTCGAGAAACTGCTGCTCTATTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAATAGAGCAGCAGTTTCTCGAGAAACTGCTGCTCTATTTCAGC-3'

Other clones with same target sequence:

(none)