Construct: shRNA TRCN0000079440

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021291.1-1566s1c1
DNA Barcode:
CCTATCATTGTGATTCTCGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc7a9 (30962)
Gene Description:
solute carrier family 7 (cationic amino acid transporter, y+ system), member 9
Transcript:
RefSeq NM_021291.1 (NON-CURRENT)
Match location:
Position 1566 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079440)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATCATTGTGATTCTCGTA
Hairpin Sequence:
5'-CCGG-CCTATCATTGTGATTCTCGTA-CTCGAG-TACGAGAATCACAATGATAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATCATTGTGATTCTCGTACTCGAGTACGAGAATCACAATGATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATCATTGTGATTCTCGTACTCGAGTACGAGAATCACAATGATAGG-3'

Other clones with same target sequence:

(none)