Construct: shRNA TRCN0000079506

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177732.2-322s1c1
DNA Barcode:
CGACAGAAATGTACCTCGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc35d1 (242585)
Gene Description:
solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1
Transcript:
RefSeq NM_177732.2 (NON-CURRENT)
Match location:
Position 322 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079506)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACAGAAATGTACCTCGAAA
Hairpin Sequence:
5'-CCGG-CGACAGAAATGTACCTCGAAA-CTCGAG-TTTCGAGGTACATTTCTGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACAGAAATGTACCTCGAAACTCGAGTTTCGAGGTACATTTCTGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACAGAAATGTACCTCGAAACTCGAGTTTCGAGGTACATTTCTGTCG-3'

Other clones with same target sequence:

(none)