Construct: shRNA TRCN0000079516

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172773.1-724s1c1
DNA Barcode:
CTACTATATGAACTGGACTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc17a5 (235504)
Gene Description:
solute carrier family 17 (anion/sugar transporter), member 5
Transcript:
RefSeq NM_172773.1 (NON-CURRENT)
Match location:
Position 724 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079516)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACTATATGAACTGGACTTA
Hairpin Sequence:
5'-CCGG-CTACTATATGAACTGGACTTA-CTCGAG-TAAGTCCAGTTCATATAGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACTATATGAACTGGACTTACTCGAGTAAGTCCAGTTCATATAGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACTATATGAACTGGACTTACTCGAGTAAGTCCAGTTCATATAGTAG-3'

Other clones with same target sequence:

(none)