Construct: shRNA TRCN0000079532

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018861.2-699s1c1
DNA Barcode:
CAGTGGACTCTTTCCTCGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc1a4 (55963)
Gene Description:
solute carrier family 1 (glutamate/neutral amino acid transporter), member 4
Transcript:
RefSeq NM_018861.2 (NON-CURRENT)
Match location:
Position 699 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079532)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTGGACTCTTTCCTCGATT
Hairpin Sequence:
5'-CCGG-CAGTGGACTCTTTCCTCGATT-CTCGAG-AATCGAGGAAAGAGTCCACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTGGACTCTTTCCTCGATTCTCGAGAATCGAGGAAAGAGTCCACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTGGACTCTTTCCTCGATTCTCGAGAATCGAGGAAAGAGTCCACTG-3'

Other clones with same target sequence:

  1. TRCN0000349526