Construct: shRNA TRCN0000079535

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008732.1-1713s1c1
DNA Barcode:
GCCTGAACTCTATCTTCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc11a2 (18174)
Gene Description:
solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
Transcript:
RefSeq NM_008732.1 (NON-CURRENT)
Match location:
Position 1713 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079535)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAACTCTATCTTCTGAA
Hairpin Sequence:
5'-CCGG-GCCTGAACTCTATCTTCTGAA-CTCGAG-TTCAGAAGATAGAGTTCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAACTCTATCTTCTGAACTCGAGTTCAGAAGATAGAGTTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAACTCTATCTTCTGAACTCGAGTTCAGAAGATAGAGTTCAGGC-3'

Other clones with same target sequence:

(none)