Construct: shRNA TRCN0000079589

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011773.1-709s1c1
DNA Barcode:
GCCAATCTGCTAATGGCCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc30a3 (22784)
Gene Description:
solute carrier family 30 (zinc transporter), member 3
Transcript:
RefSeq NM_011773.1 (NON-CURRENT)
Match location:
Position 709 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079589)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATCTGCTAATGGCCTTT
Hairpin Sequence:
5'-CCGG-GCCAATCTGCTAATGGCCTTT-CTCGAG-AAAGGCCATTAGCAGATTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATCTGCTAATGGCCTTTCTCGAGAAAGGCCATTAGCAGATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATCTGCTAATGGCCTTTCTCGAGAAAGGCCATTAGCAGATTGGC-3'

Other clones with same target sequence:

(none)