Construct: shRNA TRCN0000079621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139143.1-992s1c1
DNA Barcode:
CCACTCATGAACCGGGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc39a6 (106957)
Gene Description:
solute carrier family 39 (metal ion transporter), member 6
Transcript:
RefSeq NM_139143.1 (NON-CURRENT)
Match location:
Position 992 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTCATGAACCGGGTATTT
Hairpin Sequence:
5'-CCGG-CCACTCATGAACCGGGTATTT-CTCGAG-AAATACCCGGTTCATGAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTCATGAACCGGGTATTTCTCGAGAAATACCCGGTTCATGAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTCATGAACCGGGTATTTCTCGAGAAATACCCGGTTCATGAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000308541