Construct: shRNA TRCN0000079631

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023214.3-432s1c1
DNA Barcode:
GTGGAGAGATAATGACGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc30a7 (66500)
Gene Description:
solute carrier family 30 (zinc transporter), member 7
Transcript:
RefSeq NM_023214.3 (NON-CURRENT)
Match location:
Position 432 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079631)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGAGAGATAATGACGCTTT
Hairpin Sequence:
5'-CCGG-GTGGAGAGATAATGACGCTTT-CTCGAG-AAAGCGTCATTATCTCTCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGAGAGATAATGACGCTTTCTCGAGAAAGCGTCATTATCTCTCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGAGAGATAATGACGCTTTCTCGAGAAAGCGTCATTATCTCTCCAC-3'

Other clones with same target sequence:

  1. TRCN0000303199