Construct: shRNA TRCN0000079633

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023219.1-910s1c1
DNA Barcode:
GCCGGGACTGATATTTGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc5a4b (64454)
Gene Description:
solute carrier family 5 (neutral amino acid transporters, system A), member 4b
Transcript:
RefSeq NM_023219.1 (NON-CURRENT)
Match location:
Position 910 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079633)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGGGACTGATATTTGGAAT
Hairpin Sequence:
5'-CCGG-GCCGGGACTGATATTTGGAAT-CTCGAG-ATTCCAAATATCAGTCCCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGGGACTGATATTTGGAATCTCGAGATTCCAAATATCAGTCCCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGGGACTGATATTTGGAATCTCGAGATTCCAAATATCAGTCCCGGC-3'

Other clones with same target sequence:

(none)