Construct: shRNA TRCN0000079645

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022885.1-635s1c1
DNA Barcode:
GCTTTGGTCATGGGACTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc30a5 (69048)
Gene Description:
solute carrier family 30 (zinc transporter), member 5
Transcript:
RefSeq NM_022885.1 (NON-CURRENT)
Match location:
Position 635 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079645)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGGTCATGGGACTGTTT
Hairpin Sequence:
5'-CCGG-GCTTTGGTCATGGGACTGTTT-CTCGAG-AAACAGTCCCATGACCAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGGTCATGGGACTGTTTCTCGAGAAACAGTCCCATGACCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGGTCATGGGACTGTTTCTCGAGAAACAGTCCCATGACCAAAGC-3'

Other clones with same target sequence:

(none)