Construct: shRNA TRCN0000079654

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030696.2-1355s1c1
DNA Barcode:
GCTGGATGCAACCAAAGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc16a3 (80879)
Gene Description:
solute carrier family 16 (monocarboxylic acid transporters), member 3
Transcript:
RefSeq NM_030696.2 (NON-CURRENT)
Match location:
Position 1355 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079654)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGATGCAACCAAAGTTTA
Hairpin Sequence:
5'-CCGG-GCTGGATGCAACCAAAGTTTA-CTCGAG-TAAACTTTGGTTGCATCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGATGCAACCAAAGTTTACTCGAGTAAACTTTGGTTGCATCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGATGCAACCAAAGTTTACTCGAGTAAACTTTGGTTGCATCCAGC-3'

Other clones with same target sequence:

(none)