Construct: shRNA TRCN0000079675

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134135.1-246s1c1
DNA Barcode:
GTGGCGTATTCCTGGCTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc39a3 (106947)
Gene Description:
solute carrier family 39 (zinc transporter), member 3
Transcript:
RefSeq NM_134135.1 (CURRENT)
Match location:
Position 246 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079675)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGCGTATTCCTGGCTACAT
Hairpin Sequence:
5'-CCGG-GTGGCGTATTCCTGGCTACAT-CTCGAG-ATGTAGCCAGGAATACGCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGCGTATTCCTGGCTACATCTCGAGATGTAGCCAGGAATACGCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGCGTATTCCTGGCTACATCTCGAGATGTAGCCAGGAATACGCCAC-3'

Other clones with same target sequence:

(none)