Construct: shRNA TRCN0000079726

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022880.1-336s1c1
DNA Barcode:
CCTCGGACACTGATCAATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc29a1 (63959)
Gene Description:
solute carrier family 29 (nucleoside transporters), member 1
Transcript:
RefSeq NM_022880.1 (NON-CURRENT)
Match location:
Position 336 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079726)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGGACACTGATCAATCAT
Hairpin Sequence:
5'-CCGG-CCTCGGACACTGATCAATCAT-CTCGAG-ATGATTGATCAGTGTCCGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGGACACTGATCAATCATCTCGAGATGATTGATCAGTGTCCGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGGACACTGATCAATCATCTCGAGATGATTGATCAGTGTCCGAGG-3'

Other clones with same target sequence:

  1. TRCN0000324845