Construct: shRNA TRCN0000079734

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172271.1-1244s1c1
DNA Barcode:
CCTCTGGAAAGGTAATGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc6a17 (229706)
Gene Description:
solute carrier family 6 (neurotransmitter transporter), member 17
Transcript:
RefSeq NM_172271.1 (NON-CURRENT)
Match location:
Position 1244 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079734)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGGAAAGGTAATGTATT
Hairpin Sequence:
5'-CCGG-CCTCTGGAAAGGTAATGTATT-CTCGAG-AATACATTACCTTTCCAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGGAAAGGTAATGTATTCTCGAGAATACATTACCTTTCCAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGGAAAGGTAATGTATTCTCGAGAATACATTACCTTTCCAGAGG-3'

Other clones with same target sequence:

(none)