Construct: shRNA TRCN0000079755

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146257.1-422s1c1
DNA Barcode:
CGACTATCTTCACCACAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc29a4 (243328)
Gene Description:
solute carrier family 29 (nucleoside transporters), member 4
Transcript:
RefSeq NM_146257.1 (NON-CURRENT)
Match location:
Position 422 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079755)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTATCTTCACCACAAGTA
Hairpin Sequence:
5'-CCGG-CGACTATCTTCACCACAAGTA-CTCGAG-TACTTGTGGTGAAGATAGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTATCTTCACCACAAGTACTCGAGTACTTGTGGTGAAGATAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTATCTTCACCACAAGTACTCGAGTACTTGTGGTGAAGATAGTCG-3'

Other clones with same target sequence:

(none)