Construct: shRNA TRCN0000079758

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144902.2-3389s1c1
DNA Barcode:
CCCTACAGAAATATAGACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc35a3 (229782)
Gene Description:
solute carrier family 35 (UDP-N-acetylglucosamine (UDP-GlcNAc) transporter), member 3
Transcript:
RefSeq NM_144902.2 (NON-CURRENT)
Match location:
Position 3389 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079758)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTACAGAAATATAGACAAA
Hairpin Sequence:
5'-CCGG-CCCTACAGAAATATAGACAAA-CTCGAG-TTTGTCTATATTTCTGTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTACAGAAATATAGACAAACTCGAGTTTGTCTATATTTCTGTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTACAGAAATATAGACAAACTCGAGTTTGTCTATATTTCTGTAGGG-3'

Other clones with same target sequence:

(none)