Construct: shRNA TRCN0000079777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_205887.3-714s1c1
DNA Barcode:
CCAGTGTATGGAAACTAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc39a12 (277468)
Gene Description:
solute carrier family 39 (zinc transporter), member 12
Transcript:
RefSeq XM_205887.3 (NON-CURRENT)
Match location:
Position 714 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGTATGGAAACTAAGAT
Hairpin Sequence:
5'-CCGG-CCAGTGTATGGAAACTAAGAT-CTCGAG-ATCTTAGTTTCCATACACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGTATGGAAACTAAGATCTCGAGATCTTAGTTTCCATACACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGTATGGAAACTAAGATCTCGAGATCTTAGTTTCCATACACTGG-3'

Other clones with same target sequence:

(none)