Construct: shRNA TRCN0000079799

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016917.1-897s1c1
DNA Barcode:
CGGTTGGAATTTGGTGTCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc40a1 (53945)
Gene Description:
solute carrier family 40 (iron-regulated transporter), member 1
Transcript:
RefSeq NM_016917.1 (NON-CURRENT)
Match location:
Position 897 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079799)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTTGGAATTTGGTGTCCAT
Hairpin Sequence:
5'-CCGG-CGGTTGGAATTTGGTGTCCAT-CTCGAG-ATGGACACCAAATTCCAACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTTGGAATTTGGTGTCCATCTCGAGATGGACACCAAATTCCAACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTTGGAATTTGGTGTCCATCTCGAGATGGACACCAAATTCCAACCG-3'

Other clones with same target sequence:

(none)