Construct: shRNA TRCN0000079827

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181328.2-995s1c1
DNA Barcode:
CAGTCACTGTGGTGCTTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc25a29 (214663)
Gene Description:
solute carrier family 25 (mitochondrial carrier, palmitoylcarnitine transporter), member 29
Transcript:
RefSeq NM_181328.2 (NON-CURRENT)
Match location:
Position 995 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079827)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTCACTGTGGTGCTTACAT
Hairpin Sequence:
5'-CCGG-CAGTCACTGTGGTGCTTACAT-CTCGAG-ATGTAAGCACCACAGTGACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTCACTGTGGTGCTTACATCTCGAGATGTAAGCACCACAGTGACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTCACTGTGGTGCTTACATCTCGAGATGTAAGCACCACAGTGACTG-3'

Other clones with same target sequence:

(none)