Construct: shRNA TRCN0000079830

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144512.1-1208s1c1
DNA Barcode:
GCCAGTTTGTGTGTGTAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc6a13 (14412)
Gene Description:
solute carrier family 6 (neurotransmitter transporter, GABA), member 13
Transcript:
RefSeq NM_144512.1 (NON-CURRENT)
Match location:
Position 1208 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079830)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGTTTGTGTGTGTAGAAA
Hairpin Sequence:
5'-CCGG-GCCAGTTTGTGTGTGTAGAAA-CTCGAG-TTTCTACACACACAAACTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGTTTGTGTGTGTAGAAACTCGAGTTTCTACACACACAAACTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGTTTGTGTGTGTAGAAACTCGAGTTTCTACACACACAAACTGGC-3'

Other clones with same target sequence:

(none)