Construct: shRNA TRCN0000079841

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009320.3-1708s1c1
DNA Barcode:
CCTGGATATATGGCGGTGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc6a6 (21366)
Gene Description:
solute carrier family 6 (neurotransmitter transporter, taurine), member 6
Transcript:
RefSeq NM_009320.3 (NON-CURRENT)
Match location:
Position 1708 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079841)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGATATATGGCGGTGATA
Hairpin Sequence:
5'-CCGG-CCTGGATATATGGCGGTGATA-CTCGAG-TATCACCGCCATATATCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGATATATGGCGGTGATACTCGAGTATCACCGCCATATATCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGATATATGGCGGTGATACTCGAGTATCACCGCCATATATCCAGG-3'

Other clones with same target sequence:

(none)