Construct: shRNA TRCN0000079860

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133987.1-2350s1c1
DNA Barcode:
CTCTCTTCTATGCTATGTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc6a8 (102857)
Gene Description:
solute carrier family 6 (neurotransmitter transporter, creatine), member 8
Transcript:
RefSeq NM_133987.1 (NON-CURRENT)
Match location:
Position 2350 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079860)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTCTTCTATGCTATGTGTA
Hairpin Sequence:
5'-CCGG-CTCTCTTCTATGCTATGTGTA-CTCGAG-TACACATAGCATAGAAGAGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTCTTCTATGCTATGTGTACTCGAGTACACATAGCATAGAAGAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTCTTCTATGCTATGTGTACTCGAGTACACATAGCATAGAAGAGAG-3'

Other clones with same target sequence:

  1. TRCN0000332015