Construct: shRNA TRCN0000079863

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080853.2-2523s1c1
DNA Barcode:
CCGTCTTCATTCAGGCCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc17a6 (140919)
Gene Description:
solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Transcript:
RefSeq NM_080853.2 (NON-CURRENT)
Match location:
Position 2523 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079863)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTCTTCATTCAGGCCATTT
Hairpin Sequence:
5'-CCGG-CCGTCTTCATTCAGGCCATTT-CTCGAG-AAATGGCCTGAATGAAGACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTCTTCATTCAGGCCATTTCTCGAGAAATGGCCTGAATGAAGACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTCTTCATTCAGGCCATTTCTCGAGAAATGGCCTGAATGAAGACGG-3'

Other clones with same target sequence:

  1. TRCN0000317809