Construct: shRNA TRCN0000079873

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182959.2-2300s1c1
DNA Barcode:
CCTTCTTACAAAGATGGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc17a8 (216227)
Gene Description:
solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8
Transcript:
RefSeq NM_182959.2 (NON-CURRENT)
Match location:
Position 2300 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079873)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCTTACAAAGATGGGAAT
Hairpin Sequence:
5'-CCGG-CCTTCTTACAAAGATGGGAAT-CTCGAG-ATTCCCATCTTTGTAAGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCTTACAAAGATGGGAATCTCGAGATTCCCATCTTTGTAAGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCTTACAAAGATGGGAATCTCGAGATTCCCATCTTTGTAAGAAGG-3'

Other clones with same target sequence:

(none)