Construct: shRNA TRCN0000079903
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_133690.1-3226s1c1
- DNA Barcode:
- CCGGAGTTACTTTCTCACAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Atp1b4 (67821)
- Gene Description:
- ATPase, (Na+)/K+ transporting, beta 4 polypeptide
- Transcript:
- RefSeq NM_133690.1 (NON-CURRENT)
- Match location:
- Position 3226 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000079903)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCGGAGTTACTTTCTCACAAA
- Hairpin Sequence:
-
5'-CCGG-CCGGAGTTACTTTCTCACAAA-CTCGAG-TTTGTGAGAAAGTAACTCCGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCGGAGTTACTTTCTCACAAACTCGAGTTTGTGAGAAAGTAACTCCGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCGGAGTTACTTTCTCACAAACTCGAGTTTGTGAGAAAGTAACTCCGG-3'
Other clones with same target sequence:
(none)
