Construct: shRNA TRCN0000079904

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133690.1-247s1c1
DNA Barcode:
CGGAAATTGCAGATCGTGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp1b4 (67821)
Gene Description:
ATPase, (Na+)/K+ transporting, beta 4 polypeptide
Transcript:
RefSeq NM_133690.1 (NON-CURRENT)
Match location:
Position 247 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079904)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAATTGCAGATCGTGAAT
Hairpin Sequence:
5'-CCGG-CGGAAATTGCAGATCGTGAAT-CTCGAG-ATTCACGATCTGCAATTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAATTGCAGATCGTGAATCTCGAGATTCACGATCTGCAATTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAATTGCAGATCGTGAATCTCGAGATTCACGATCTGCAATTTCCG-3'

Other clones with same target sequence:

(none)