Construct: shRNA TRCN0000079917

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019810.2-932s1c1
DNA Barcode:
CATGAAAGCTATTCCAACTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc5a1 (20537)
Gene Description:
solute carrier family 5 (sodium/glucose cotransporter), member 1
Transcript:
RefSeq NM_019810.2 (NON-CURRENT)
Match location:
Position 932 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079917)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGAAAGCTATTCCAACTAA
Hairpin Sequence:
5'-CCGG-CATGAAAGCTATTCCAACTAA-CTCGAG-TTAGTTGGAATAGCTTTCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGAAAGCTATTCCAACTAACTCGAGTTAGTTGGAATAGCTTTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGAAAGCTATTCCAACTAACTCGAGTTAGTTGGAATAGCTTTCATG-3'

Other clones with same target sequence:

(none)