Construct: shRNA TRCN0000079970

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009262.1-235s1c1
DNA Barcode:
CAACGCCAATTTCCTAGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spock1 (20745)
Gene Description:
sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1
Transcript:
RefSeq NM_009262.1 (NON-CURRENT)
Match location:
Position 235 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079970)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACGCCAATTTCCTAGACAA
Hairpin Sequence:
5'-CCGG-CAACGCCAATTTCCTAGACAA-CTCGAG-TTGTCTAGGAAATTGGCGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACGCCAATTTCCTAGACAACTCGAGTTGTCTAGGAAATTGGCGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACGCCAATTTCCTAGACAACTCGAGTTGTCTAGGAAATTGGCGTTG-3'

Other clones with same target sequence:

(none)