Construct: shRNA TRCN0000080004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177028.2-154s1c1
DNA Barcode:
CCAAAGAATACGCTCTGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Oacyl (319888)
Gene Description:
O-acyltransferase like
Transcript:
RefSeq NM_177028.2 (NON-CURRENT)
Match location:
Position 154 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGAATACGCTCTGAGAA
Hairpin Sequence:
5'-CCGG-CCAAAGAATACGCTCTGAGAA-CTCGAG-TTCTCAGAGCGTATTCTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGAATACGCTCTGAGAACTCGAGTTCTCAGAGCGTATTCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGAATACGCTCTGAGAACTCGAGTTCTCAGAGCGTATTCTTTGG-3'

Other clones with same target sequence:

(none)