Construct: shRNA TRCN0000080050

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009691.1-1625s1c1
DNA Barcode:
GAGCGGATATGGACCAATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aplp2 (11804)
Gene Description:
amyloid beta (A4) precursor-like protein 2
Transcript:
RefSeq NM_009691.1 (NON-CURRENT)
Match location:
Position 1625 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080050)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCGGATATGGACCAATTTA
Hairpin Sequence:
5'-CCGG-GAGCGGATATGGACCAATTTA-CTCGAG-TAAATTGGTCCATATCCGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCGGATATGGACCAATTTACTCGAGTAAATTGGTCCATATCCGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCGGATATGGACCAATTTACTCGAGTAAATTGGTCCATATCCGCTC-3'

Other clones with same target sequence:

(none)