Construct: shRNA TRCN0000080054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173051.1-833s1c1
DNA Barcode:
CTGTGTCAAATTGCCCAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Serpinb1c (380839)
Gene Description:
serine (or cysteine) peptidase inhibitor, clade B, member 1c
Transcript:
RefSeq NM_173051.1 (NON-CURRENT)
Match location:
Position 833 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTGTCAAATTGCCCAAATT
Hairpin Sequence:
5'-CCGG-CTGTGTCAAATTGCCCAAATT-CTCGAG-AATTTGGGCAATTTGACACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTGTCAAATTGCCCAAATTCTCGAGAATTTGGGCAATTTGACACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTGTCAAATTGCCCAAATTCTCGAGAATTTGGGCAATTTGACACAG-3'

Other clones with same target sequence:

(none)