Construct: shRNA TRCN0000080270

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023689.2-568s1c1
DNA Barcode:
GCAAACTAGAATATCAGGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spock3 (72902)
Gene Description:
sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3
Transcript:
RefSeq NM_023689.2 (NON-CURRENT)
Match location:
Position 568 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080270)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAACTAGAATATCAGGCAT
Hairpin Sequence:
5'-CCGG-GCAAACTAGAATATCAGGCAT-CTCGAG-ATGCCTGATATTCTAGTTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAACTAGAATATCAGGCATCTCGAGATGCCTGATATTCTAGTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAACTAGAATATCAGGCATCTCGAGATGCCTGATATTCTAGTTTGC-3'

Other clones with same target sequence:

(none)