Construct: shRNA TRCN0000080310

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029733.1-454s1c1
DNA Barcode:
TGGTGGTTGTTTCCTCCACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2010005H15Rik (76770)
Gene Description:
RIKEN cDNA 2010005H15 gene
Transcript:
RefSeq NM_029733.1 (NON-CURRENT)
Match location:
Position 454 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080310)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGGTTGTTTCCTCCACAT
Hairpin Sequence:
5'-CCGG-TGGTGGTTGTTTCCTCCACAT-CTCGAG-ATGTGGAGGAAACAACCACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGGTTGTTTCCTCCACATCTCGAGATGTGGAGGAAACAACCACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGGTTGTTTCCTCCACATCTCGAGATGTGGAGGAAACAACCACCA-3'

Other clones with same target sequence:

(none)