Construct: shRNA TRCN0000080335

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011340.2-1182s1c1
DNA Barcode:
CCCGCTAGACTATCACCTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Serpinf1 (20317)
Gene Description:
serine (or cysteine) peptidase inhibitor, clade F, member 1
Transcript:
RefSeq NM_011340.2 (NON-CURRENT)
Match location:
Position 1182 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080335)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGCTAGACTATCACCTTAA
Hairpin Sequence:
5'-CCGG-CCCGCTAGACTATCACCTTAA-CTCGAG-TTAAGGTGATAGTCTAGCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGCTAGACTATCACCTTAACTCGAGTTAAGGTGATAGTCTAGCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGCTAGACTATCACCTTAACTCGAGTTAAGGTGATAGTCTAGCGGG-3'

Other clones with same target sequence:

(none)