Construct: shRNA TRCN0000080487

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144899.2-2430s1c1
DNA Barcode:
GTTCGGTGTGTTGGCAGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adamtsl4 (229595)
Gene Description:
ADAMTS-like 4
Transcript:
RefSeq NM_144899.2 (NON-CURRENT)
Match location:
Position 2430 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080487)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCGGTGTGTTGGCAGTAAT
Hairpin Sequence:
5'-CCGG-GTTCGGTGTGTTGGCAGTAAT-CTCGAG-ATTACTGCCAACACACCGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCGGTGTGTTGGCAGTAATCTCGAGATTACTGCCAACACACCGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCGGTGTGTTGGCAGTAATCTCGAGATTACTGCCAACACACCGAAC-3'

Other clones with same target sequence:

(none)